Development of a nicking endonuclease-assisted method for the purification of minicircles
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- 作者：Clá ; udia P.A. Alves ; Michaela &Scaron ; imčí ; ková ; Liliana Brito ; Gabriel A. Monteiro ; Duarte Miguel F. Prazeres ; miguelprazeres@tecnico.ulisboa.pt" class="auth_mail" title="E-mail the corresponding author
- 关键词：Gene therapy ; Hydrophobic interaction chromatography ; Minicircle ; Nicking endonuclease ; Purification
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：22 April 2016
- 年：2016
- 卷：1443
- 期：Complete
- 页码：136-144
- 全文大小：1346 K
文摘
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A process is described to improve the purification of supercoiled minicircles.
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Supercoiled miniplasmid impurities are enzymatically relaxed in vitro.
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Topoisomers are separated by hydrophobic interaction chromatography.