Synthesis and secretion of macrophage colony stimulating factor by mature human monocytes and human monocytic THP-1 cells induced by human serum albumin derivatives modified with methylglyoxal and glu

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• 作者：Abordo ; Evelyn A. ; Westwood ; Marie E. ; Thornalley ; Paul J.
• 关键词：Macrophage colony stimulating factor (M-CSF) ; Monocyte ; Methylglyoxal ; Glycation ; Advanced glycation endproduct ; Diabetic complications
• 刊名：Immunology Letters
• 出版年：1996
• 出版时间：October, 1996
• 年：1996
• 卷：53
• 期：1
• 页码：7-13
• 全文大小：664 K

文摘

Human serum albumin minimally-modified by methylglyoxal (MG_min-HSA) stimulated the synthesis and secretion of macrophage-colony stimulating factor (M-CSF) by mature human monocytes in vitro. Human serum albumin minimally-modified by glucose-derived advanced glycation endproducts (AGE_min-HSA) and human serum albumin highly-modified by glucose-derived advanced glycation endproducts (AGE-HSA) stimulated much lower secretion of M-CSF from human monocytes than did MG_min-HSA. MG_min-HSA and AGE-HSA but not AGE_min-HSA also stimulated the growth of human monocytic THP-1 cells in vitro which was inhibited by polyclonal antibodies to human M-CSF. For MG_min-HSA, the median growth stimulatory concentration EC₅₀ value was 0.24 ± 0.07 μM and the maximal increase in cell growth was 36 % of control cell growth (n = 24). Similar induction of secretion of M-CSF from monocytes in vivo may contribute to atherosclerosis in macro- and micro-angiopathy, particularly in the development of diabetic complications.