A novel Cry9Aa with increased toxicity for Spodoptera exigua (H眉bner)
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- 作者：Samir Naimov ; Radostina Nedyalkova ; Nikola Staykov ; Mieke Weemen-Hendriks ; Ivan Minkov ; Ruud A. de Maagd
- 关键词：Spodoptera exigua (Hü ; bner) (Lepidoptera ; Noctuidae) ; Bacillus thuringiensis ; Cry9Aa5
- 刊名：Journal of Invertebrate Pathology
- 出版年：January, 2014
- 年：2014
- 卷：115
- 期：Complete
- 页码：99-101
- 全文大小：323 K
文摘
Cry9Aa, produced by Bacillus thuringiensis is reported to be not active against Spodoptera exigua (beet armyworm). In this study we have cloned a new cry9Aa5 gene encoding a protoxin with increased activity against S. exigua as compared to Cry9Aa1. When aligned to Cry9Aa1, four amino acid substitutions in domain I and one substitution in the C-terminal protein extension of Cry9Aa5 were identified. Toxicity of Cry9Aa5, produced in recombinant Escherichia coli was assessed and compared to the activity of Cry9Aa1, produced under the same conditions.