Chitosan-polyvinylalcohol nanofibers were electrospun on screen-printed electrodes.
Recombinant acetylcholinesterase was immobilized on nanofibers by glutaraldehyde coupling.
Pirimiphos-methyl insecticide was detected with a LOD of 0.2 nM.
The biosensor was used for pirimiphos-methyl determination in olive oil, 100% recovery rate was achieved.