Biosensor based on electrospun blended chitosan-poly (vinyl alcohol) nanofibrous enzymatically sensitized membranes for pirimiphos-methyl detection in olive oil
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- 作者：A.Y. El-Moghazya ; E.A. Solimana ; H.Z. Ibrahimb ; J.-L. Martyc ; G. Istambouliec ; T. Noguerc ;
- 关键词：Biosensor ; Nanofibers ; Electrospinning ; Genetically-Modified Acetylcholinesterase ; Organophosphorus Pesticides ; Olive Oil
- 刊名：Talanta
- 出版年：2016
- 出版时间：1 August 2016
- 年：2016
- 卷：155
- 期：Complete
- 页码：258-264
- 全文大小：1966 K
文摘
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Chitosan-polyvinylalcohol nanofibers were electrospun on screen-printed electrodes.
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Recombinant acetylcholinesterase was immobilized on nanofibers by glutaraldehyde coupling.
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Pirimiphos-methyl insecticide was detected with a LOD of 0.2 nM.
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The biosensor was used for pirimiphos-methyl determination in olive oil, 100% recovery rate was achieved.