CD14 is not involved in the uptake of synthetic CpG oligonucleotides

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• 作者：Jessica Li^a ; ^b ; Fatma Ahmet^a ; Meredith O&rsquo ; Keeffe^a ; ^c ; Mireille H. Lahoud^a ; ^c ; William R. Heath^b ; Irina Caminschi^a ; ^b ; ^c ; ^{irina.caminschi@monash.edu}
• 关键词：EDTA ; ethylenediamine tetra-acetic acid disodium salt ; PE ; phycoerythrin ; SA ; streptavidin ; PBS ; phosphate buffered saline ; i.v. ; intravenous ; LPS ; lipopolysaccharide
• 刊名：Molecular Immunology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：81
• 期：Complete
• 页码：52-58
• 全文大小：1409 K
• 卷排序：81

文摘

We show that CD14 does not bind to A-, B-, and C-Class CpG ODN, although it unequivocally binds its known ligand LPS. Our studies dispute the claim that CD14 is involved in CpG ODN capture. Consistent with the inability of CD14 to bind CpG ODN in vitro, our in vivo data shows CD14 was not required for the activation of CD8+ DC, or systemic cytokine production by the broader cellular population in response to CpG ODN.