Solanum incanum and S. heteracanthum as sources of biologically active steroid glycosides: Confirmation of their synonymy

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• 作者：Mahenina Jaovita Manase ; Anne-Claire Mitaine-Offer ; David Pertuit ; Tomofumi Miyamoto ; Chiaki Tanaka ; St¨¦phanie Delemasure ; Patrick Dutartre ; Jean-Fran?ois Mirjolet ; Olivier Duchamp ; Marie-Aleth Lacaille-Dubois
• 关键词：Solanum incanum ; Solanum heteracanthum ; Steroidal saponins ; Steroidal glycoalkaloid ; Cytotoxicity ; Antioxidant
• 刊名：Fitoterapia
• 出版年：2012
• 出版时间：September, 2012
• 年：2012
• 卷：83
• 期：6
• 页码：1115-1119
• 全文大小：290 K

文摘

A new spirostanol saponin (1), along with four known saponins, dioscin (2), protodioscin (3), methyl-protodioscin (4), and indioside D (5), and one known steroid glycoalkaloid solamargine (6) were isolated from the two synonymous species, Solanum incanum and S. heteracanthum. The structure of the new saponin was established as (23S,25R)-spirost-5-en-3¦Â,23-diol 3-O-{¦Â-d-xylopyranosyl-(1¡ú2)-O-¦Á-l-rhamnopyranosyl-(1¡ú4)-[O-¦Á-l-rhamnopyranosyl-(1¡ú2)]-¦Â-d-glucopyranoside}, by using a combination of 1D and 2D NMR techniques including ¹H, ¹³C, COSY, TOCSY, NOESY, HSQC and HMBC experiments and by mass spectrometry. The compounds 1, 3, 4 and 5 were evaluated for cytotoxicity against five cancer cell lines and for antioxidant and cytoprotective activity.