Association between methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism and risk of ischemic stroke in North Indian population: A hospital based case-control study

详细信息    查看全文

• 作者：Amit Kumar ; Shubham Misra ; Anjali Hazarika ; Pradeep Kumar ; Ram Sagar ; Abhishek Pathak ; Kamalesh Chakravarty ; Kameshwar Prasad ; ^{kp0704@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ischemic stroke ; Polymorphisms ; Methylene tetrahydrofolate reductase ; North India ; Cerebrovascular disease
• 刊名：Egyptian Journal of Medical Human Genetics
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：17
• 期：4
• 页码：359-365
• 全文大小：514 K

文摘

The aim of the present case–control study was to determine the association between methylene tetrahydrofolate reductase (MTHFR) C677T (rs1801133) gene polymorphism and risk of ischemic stroke (IS) in North Indian population.

Methods

Patients with IS and age-sex matched controls were recruited from Neurology Outpatient Department and Ward of All India Institute of Medical Sciences, New Delhi, India. Genotyping was performed by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) method. PCR–RFLP results of nine randomly selected samples were confirmed by DNA sequencing. Genotypic and allelic distributions were compared between cases and controls. Statistical analysis was done by STATA, version 13.0 software.

Results

Hypertension, diabetes, dyslipidemia, low socioeconomic status and family history of stroke were found to have an independent association with the risk of IS after adjusting for potential confounding factors. Mean age of cases and controls were 52.83 ± 12.59 and 50.97 ± 12.70 years. Multivariate logistic regression analysis showed an independent association between MTHFR C677T gene polymorphism and risk of IS (OR 1.91; 95% CI 1.07–3.41; p = 0.028) under dominant model [CT + TT vs. CC]. MTHFR C677T gene polymorphism was found to be independently associated with risk of small vessel disease (SVD) after adjustment for potential confounding factors [OR 2.51; 95% CI 1.30–4.85; p = 0.006] under the dominant model.

Conclusion

Findings of the present study suggest that MTHFR C677T gene polymorphism might be a risk factor of IS mainly for SVD subtypes of IS in North Indian population. Further large prospective studies are required to confirm these findings.