Allogeneic/xenogeneic transplantation of peptide-labeled mitochondria in Parkinson's disease: restoration of mitochondria functions and attenuation of 6-hydroxydopamine-induced neurotoxicity

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• 作者：Jui-Chih Chang^a ; Shey-Lin Wu^b ; Ko-Hung Liu^a ; Ya-Hui Chen^a ; Chieh-Sen Chuang^b ; Fu-Chou Cheng^c ; Hong-Lin Su^d ; Yau-Huei Wei^e ; ^f ; Shou-Jen Kuo^g ; Chin-San Liu^a ; ^b ; ^h ; ^{liu48111@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：MFB ; medial forebrain bundle ; Mito ; allogeneic mitochondria ; 6-OHDA ; 6-hydroxydopamine ; PD ; Parkinson's disease ; PMD ; peptide-mediated delivery of allogeneic mitochondria ; SN ; substantia nigra ; SNc ; substantia nigra pars compacta ; ST ; striatum ; TH ; tyrosine hydroxylase ; xPMD ; peptide-mediated delivery of xenogeneic mitochondria
• 刊名：Translational Research
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：170
• 期：Complete
• 页码：40-56.e3
• 全文大小：4654 K

文摘

Although restoration of mitochondrial function in mitochondrial diseases through peptide-mediated allogeneic mitochondrial delivery (PMD) has been demonstrated in vitro, the in vivo therapeutic efficacy of PMD in Parkinson's disease (PD) has yet to be determined. In this study, we compared the functionality of mitochondrial transfer with or without Pep-1 conjugation in neurotoxin (6-hydroxydopamine, 6-OHDA)-induced PC12 cells and PD rat models. We injected mitochondria into the medial forebrain bundle (MFB) of the PD rats after subjecting the nigrostriatal pathway to a unilateral 6-OHDA lesion for 21 days, and we verified the effectiveness of the mitochondrial graft in enhancing mitochondrial function in the soma of the substantia nigra (SN) neuron through mitochondrial transport dynamics in the nigrostriatal circuit. The result demonstrated that only PMD with allogeneic and xenogeneic sources significantly sustained mitochondrial function to resist the neurotoxin-induced oxidative stress and apoptotic death in the rat PC12 cells. The remaining cells exhibited a greater capability of neurite outgrowth. Furthermore, allogeneic and xenogeneic transplantation of peptide-labeled mitochondria after 3 months improved the locomotive activity in the PD rats. This increase was accompanied by a marked decrease in dopaminergic neuron loss in the substantia nigra pars compacta (SNc) and consistent enhancement of tyrosine hydroxylase–positive immunoreaction of dopaminergic neurons in the SNc and striatum. We also observed that in the SN dopaminergic neuron in the treated PD rats, mitochondrial complex I protein and mitochondrial dynamics were restored, thus ameliorating the oxidative DNA damage. Moreover, we determined signal translocation of graft allogeneic mitochondria from the MFB to the calbindin-positive SN neuron, which demonstrated the regulatory role of mitochondrial transport in alleviating 6-OHDA–induced degeneration of dopaminergic neurons.