Screening and discovery of lineage-specific mitosomal membrane proteins in Entamoeba histolytica

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• 作者：Herbert J. Santos^a ; ^b ; Kenichiro Imai^c ; Yuki Hanadate^a ; ^b ; Yoshinori Fukasawa^c ; Toshiyuki Oda^c ; Fumika Mi-ichi^b ; ^d ; Tomoyoshi Nozaki^a ; ^b ; ^{nozaki@nih.go.jp}
• 关键词：MRO ; mitochondrion-related organelle ; Tom ; translocase of the outer mitochondrial membrane ; PAPS ; 3&prime ; -phosphoadenosine-5&prime ; -phosphosulfate ; APSK ; adenosine-5&prime ; -phosphokinase ; Cpn60 ; chaperonin60 ; TMD ; transmembrane domain ; IMP ; inner membrane protein ; SVM ; support vector machine ; IFA ; immunofluorescence assay
• 刊名：Molecular and Biochemical Parasitology
• 出版年：2016
• 出版时间：September-October 2016
• 年：2016
• 卷：209
• 期：1-2
• 页码：10-17
• 全文大小：2486 K
• 卷排序：209

文摘

We developed a TMD predictor to discover mitosomal membrane proteins. It predicted 25 candidate proteins that are specific to Entamoeba mitosomes. Two representatives, EHI_170120 and EHI_099350, were localized to mitosomes. EHI_170120 appears to be integrated to the mitosomal membrane. EHI_099350 is localized to the matrix of mitosomes that seem to lack Cpn60.