Transforming growth factor-β₁ as an apoptotic agent in L1210 leukemic cells.

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• 作者：Motyl ; T. ; Grzelkowska ; K. ; Kasterka ; M. ; Skierski ; J. ; Ostrowski ; J.
• 刊名：Regulatory Peptides
• 出版年：1996
• 出版时间：July 15, 1996
• 年：1996
• 卷：64
• 期：1-3
• 页码：132
• 全文大小：82.5 K

文摘

TGF-β₁ exerts apoptotic, necrotic, and mitoinhibitory effects in L1210 leukemic cells. Apoptosis is induced at low TGF-β₁ concentrations (0.001 - 0.1 ng/ml), whereas mitoinhibition at concentrations exceeding 1 ng/ml. Necrosis is probably a secondary to apoptosis form of death, since TGF-β₁ is not considered as a cytotoxic agent. Apoptosis is manifested by cell shrinkage, chromatin condensation and margination, nuclear pycnosis and fragmentation. Characteristic “DNA ladder” resulting from its internucleosomal fragmentation (multiples of 180 - 200 bp) was visualised by gel electrophoresis. Flow cytometry analysis demonstrated that apoptosis affects mainly cells in G₁ phase. The induction of apoptosis by TGF-β₁ depends on the supply of growth and trophic factors present in fetal calf serum (FCS). FCS deprivation and bovine serum albumin supplementation enhances cell susceptibility to TGF-β₁-induced apoptosis.