Using Electrochemical SERS to Measure the Redox Potential of Drug Molecules Bound to dsDNA—a Study of Mitoxantrone

详细信息    查看全文

• 作者：Marta Meneghello^b ; Evanthia Papadopoulou^a ; Paolo Ugo^b ; ^{ugo@unive.it" class="auth_mail" title="E-mail the corresponding author} ; Philip N. Bartlett^a ; ^{p.n.bartlett@soton.ac.uk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Electrochemical SERS ; Mitoxantrone ; dsDNA ; Redox reaction
• 刊名：Electrochimica Acta
• 出版年：2016
• 出版时间：1 January 2016
• 年：2016
• 卷：187
• 期：Complete
• 页码：684-692
• 全文大小：1483 K

文摘

Interaction with DNA plays an important role in the biological activity of some anticancer drug molecules. In this paper we show that electrochemical surface enhanced Raman spectroscopy at sphere segment void gold electrodes can be used as a highly sensitive technique to measure the redox potential of the anticancer drug mitoxantrone bound to dsDNA. For this system we show that we can follow the redox reaction of the bound molecule and can extract the redox potential for the molecule bound to dsDNA by deconvolution of the SER spectra recorded as a function of electrode potential. We find that mitoxantrone bound to dsDNA undergoes a 2 electron, 1 proton reduction and that the redox potential (-0.87 V vs. Ag/AgCl at pH 7.2) is shifted approximately 0.12 V cathodic of the corresponding value at a glassy carbon electrode. Our results also show that the reduced form of mitoxantrone remains bound to dsDNA and we are able to use the deconvoluted SER spectra of the reduced mitoxantrone as a function of electrode potential to follow the electrochemically driven melting of the dsDNA at more negative potentials.