- 作者：Sachiyo Nishimura ; Hiroyasu Ishikura ; Maho Matsunami ; Yui Shinozaki ; Fumiko Sekiguchi ; Mitsuhide Naruse ; Taisuke Kitamura ; Ryukichi Akashi ; Kenji Matsumura ; Atsufumi Kawabata
- 关键词：Capsazepine ; PAR2 ; Proteinase inhibitor ; Pancreatitis ; Transient receptor potential vanilloid-1 ; Trypsin ; Visceral pain
- 刊名：Life Sciences
- 出版年：2010
- 出版时间：20 November 2010
- 年：2010
- 卷：87
- 期：19-22
- 页码：643-650
- 全文大小：1634 K
AimsProteinase-activated receptor-2 (PAR2) and transient receptor potential vanilloid-1 (TRPV1) are co-localized in the primary afferents, and the trans-activation of TRPV1 by PAR2 activation is involved in processing of somatic pain. Given evidence for contribution of PAR2 to pancreatic pain, the present study aimed at clarifying the involvement of TRPV1 in processing of pancreatic pain by the proteinase/PAR2 pathway in mice.Main methods
Acute pancreatitis was created by repeated administration of cerulein in conscious mice, and the referred allodynia/hyperalgesia was assessed using von Frey filaments. Injection of PAR2 agonists into the pancreatic duct was achieved in anesthetized mice, and expression of Fos in the spinal cord was determined by immunohistochemistry.
Key findings
The established referred allodynia/hyperalgesia following cerulein treatment was abolished by post-treatment with nafamostat mesilate, a proteinase inhibitor, and with capsazepine, a TRPV1 antagonist, in mice. Injection of trypsin, an endogenous PAR2 agonist, or SLIGRL-NH2, a PAR2-activating peptide, into the pancreatic duct caused expression of Fos protein in the spinal superficial layers at T8-T10 levels in the mice. The spinal Fos expression caused by trypsin and by SLIGRL-NH2 was partially blocked by capsazepine, the former effect abolished by nafamostat mesilate.
Significance
Our data thus suggest that the proteinase/PAR2/TRPV1 cascade might impact pancreatic pain, in addition to somatic pain, and play a role in the maintenance of pancreatitis-related pain in mice.