Histamine stimulates production of osteoclast differentiation factor/receptor activator of nuclear factor-κB ligand by osteoblasts

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• 作者：Deyama ; Yoshiaki ; Kikuiri ; Takashi ; Ohnishi ; Gen-ichi ; Feng ; Yi-Gang ; Takeyama ; Sadaaki ; Hatta ; Mitsutoki ; et. al.
• 关键词：Histamine ; Histamine receptor ; Osteoclast ; Osteoblast ; Stromal cell ; Osteoclast differentiation factor/receptor activator of nuclear factor-κ ; B ligand (ODF/RANKL)
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2002
• 出版时间：October 25, 2002
• 年：2002
• 卷：298
• 期：2
• 页码：240-246
• 全文大小：202 K

文摘

Histamine H₁,H₂, and H₃ receptors are expressed by osteoblastic MC3T3-E1 (E1) cells derived from mouse calvaria. Expression of the osteoclast differentiation factor (ODF)/receptor activator of nuclear factor-κB ligand (RANKL) transcript was induced in E1 cells and bone marrow stromal cells (ST2). Histamine markedly increased the steady-state level of ODF/RANKL mRNA in a dose-dependent manner. The effect of histamine on expression of ODF/RANKL mRNA by E1 cells was transient, with a peak at 6h. Western blot analysis revealed that histamine increased production of ODF/RANKL protein by E1 cells at 12h. In cocultures of E1 cells and mouse bone marrow cells, histamine stimulated osteoclastogenesis in the presence of 1,25-dihydroxyvitamin D₃ and this effect was blocked by preincubation with neutralizing antibody against ODF/RANKL. These results suggest that histamine regulates osteoclastogenesis, at least in part, through induction of ODF/RANKL expression by osteoblasts and bone marrow stromal cells.