XLMR protein related to neurite extension (Xpn/KIAA2022) regulates cell-cell and cell-matrix adhesion and migration
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- 作者：Takuya Magome ; Tsuyoshi Hattori ; Manabu Taniguchi ; Toshiko Ishikawa ; Shingo Miyata ; Kohei Yamada ; Hironori Takamura ; Shinsuke Matsuzaki ; Akira Ito ; Masaya Tohyama ; Taiichi Katayama
- 关键词：XLMR ; X-linked mental retardation ; Xpn ; XLMR protein related to neurite extension ; GAPDH ; glyceraldehyde 3-phosphate dehydrogenase ; GFP ; green fluorescent protein ; RFP ; red fluorescent protein ; DAPI ; 6-diamidino-2-phenylindole dihydrochloride ; NGF ; nerve growth factor ; qRT-PCR ; quantitative real-time PCR ; ATRX ; alpha thalassemia/mental retardation syndrome X-linked ; CP ; cortical plate ; DCX ; doublecortin ; TUBA1A ; tubulin alpha1A ; VLDLR ; very low density lipoprotein receptor ; ARX ; aristaless relat
- 刊名：Neurochemistry International
- 出版年：November, 2013
- 年：2013
- 卷：63
- 期：6
- 页码：561-569
- 全文大小：2547 K
文摘
X-linked mental retardation (XLMR) is a common cause of moderate to severe intellectual disability in males. XLMR protein related to neurite extension (Xpn, also known as KIAA2022) has been implicated as a gene responsible for XLMR in humans. Although Xpn is highly expressed in the developing brain and is involved in neurite outgrowth in PC12 cells and neurons, little is known about the functional role of Xpn. Here, we show that Xpn regulates cell-cell and cell-matrix adhesion and migration in PC12 cells. Xpn knockdown enhanced cell-cell and cell-matrix adhesion mediated by N-cadherin and 尾1-integrin, respectively. N-Cadherin and 尾1-integrin expression at the mRNA and protein levels was significantly increased in Xpn knockdown PC12 cells. Furthermore, overexpressed Xpn protein was strongly expressed in the nuclei of PC12 and 293T cells. Finally, depletion of Xpn perturbed cellular migration by enhancing N-cadherin and 尾1-integrin expression in a PC12 cell wound healing assay. We conclude that Xpn regulates cell-cell and cell-matrix adhesion and cellular migration by regulating the expression of adhesion molecules.