Dual effects of interleukin-1β on N-methyl--aspartate-induced retinal neuronal death in rat eyes
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- 作者：Kido ; Noriaki ; Inatani ; Masaru ; Honjo ; Megumi ; Yoneda ; Shinji ; Hara ; Hideaki ; Miyawaki ; Nobuaki ; et. al.
- 关键词：Neurotransmitters ; modulators ; transporters ; and receptors ; Excitatory amino acids ; excitotoxicity ; Retinal ganglion cell ; Glutamate ; Excitatory amino acid ; Interleukin-1β ; Cell death ; N-Methyl-d-aspartate
- 刊名：Brain Research
- 出版年：2001
- 出版时间：August 10, 2001
- 年：2001
- 卷：910
- 期：1-2
- 页码：153-162
- 全文大小：961 K
文摘
In this study we determine if interleukin-1β (IL-1β) modulates N-methyl-d-aspartate (NMDA)-induced retinal damage. Sprague–Dawley rats were anesthetized with inhalation of halothane, after which a single injection of 5 μl of IL-1β (0.1 to 10 ng/eye) (and/or IL-1 receptor antagonist (IL-1ra)) for experimental eyes was administered. Two days later (or simultaneously), NMDA (20 nmol) was injected into the vitreous space. One week later, each eye was enucleated and transverse sections were subjected to morphometric analysis. Enzyme-linked immunosorbent assay (ELISA) was conducted for the determination of IL-1β levels in retina. Immunohistochemical and immunoblot studies were also performed. In eyes that received an intravitreal injection of IL-1β (0.1 to 10 ng/eye), significant thinning of the inner plexiform layer (IPL) was observed (P<0.05). Immunohistochemical and ELISA studies demonstrated upregulated expression of IL-1β in retinas that had undergone NMDA injection. Treatment with 10 ng of IL-1ra induced a protective effect against NMDA-induced retinal damage. Pretreatment with IL-1β induced a significant protective effect on NMDA-induced retinal damage. Our studies suggest that IL-1β induces neuronal cell death directly, as shown by the protective effects of IL-1ra, but has a protective effect on NMDA-induced retinal damage indirectly after an incubation time of at least 2 days.