Suppression of the hyperpolarization-activated inward current contributes to the inhibitory actions of propofol on rat CA1 and CA3 pyramidal neurons

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• 作者：Higuchi ; Hitoshi ; Funahashi ; Makoto ; Miyawaki ; Takuya ; Mitoh ; Yoshihiro ; Kohjitani ; Atsushi ; et. al.
• 关键词：Propofol ; I_H ; Brain slice ; Rats
• 刊名：Neuroscience Research
• 出版年：2003
• 出版时间：April, 2003
• 年：2003
• 卷：45
• 期：4
• 页码：459-472
• 全文大小：431 K

文摘

Intracellular and field potential recordings were taken from the hippocampal CA1 and CA3 neurons in rat brain slices to investigate the effects of 2,6 di-isopropylphenol (propofol) on the neuronal excitability during GABA_A-C1 channel blockade by picrotoxin (100 μM). Propofol produced a membrane hyperpolarization and an inhibition of the magnitude of the ‘voltage sag’ that was mediated by the activation of a hyperpolarization-activated inward current (I_H). Propofol (>100 μM) decreased the spontaneous discharge rate of epileptiform burst responses in CA1 neurons up to 38±6 % of the control level. Propofol also markedly reduced the duration of both spontaneous and evoked epileptiform burst responses. A propofol-induced decrease in the spontaneous discharge rate in CA3 neurons was coincident with that in CA1 neurons. The effects of propofol on the membrane potential and spontaneous discharge rate but not on the duration of burst responses were duplicated by ZD7288 (potent selective antagonist for I_H channels), indicating that the blockade of I_H significantly contributes to reduction of cell's excitability. The present study suggests that various actions including suppressive effects on I_H contribute to the anesthetic and anti-convulsant properties of propofol.