Proposal of Helicobacter canicola sp. nov., previously identified as Helicobacter cinaedi, isolated from canines

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• 作者：Yoshiaki Kawamura^a ; ^{kawamura@dpc.agu.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Junko Tomida^a ; ^{jtomida@dpc.agu.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Tohru Miyoshi-Akiyama^b ; ^{takiyam@ri.ncgm.go.jp" class="auth_mail" title="E-mail the corresponding author} ; Tatsuya Okamoto^c ; ^{tatton.ncgm@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Masashi Narita^d ; ^{masashi.narita@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Katsuhiko Hashimoto^e ; ^{katsu0419@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Margo Cnockaert^f ; ^{Margo.Cnockaert@UGent.be" class="auth_mail" title="E-mail the corresponding author} ; Peter Vandamme^f ; ^{Peter.Vandamme@UGent.be" class="auth_mail" title="E-mail the corresponding author} ; Yuji Morita^a ; ^{yujmor@dpc.agu.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Tomohiro Sawa^g ; ^{sawat@kumamoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Takaaki Akaike^h ; ^{takaike@med.tohoku.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Canine ; DNA&ndash ; DNA hybridization ; In silico DNA similarity analysis ; Average nucleotide identity (ANI) ; DNA gyrase ; Heat shock protein ; 16S rRNA ; 23S rRNA
• 刊名：Systematic and Applied Microbiology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：39
• 期：5
• 页码：307-312
• 全文大小：913 K

文摘

During the course of our taxonomic investigation of Helicobacter cinaedi, it was realized that the strains isolated from dogs, which have been identified as H. cinaedi, showed different biochemical traits than did the isolates obtained from humans. None of the three dog isolates could reduce nitrate to nitrite, whereas all of the human H. cinaedi isolates could do so. The dog isolates showed a strong positive alkaline phosphatase reaction and could grow at 42 °C, however the human isolates showed negative to very weak responses to those tests. The GyrA protein based phylogenetic analysis showed that the three isolates from dogs formed a slightly distinct cluster from the human isolate cluster. Phylogenetic analysis of the 16S rRNA, 23S rRNA, gyrB, and hsp60 gene sequences further confirmed that the dog isolates differed from the human H. cinaedi isolate cluster. The whole-genome in silico DNA similarities of each isolate based on their full genome sequences revealed that the isolates from dogs shared more than 94.9% ANIb (average nucleotide identity based on BLAST), while 94.0% ANIb were found between the isolates from dogs and the humans, including the H. cinaedi type strain ATCC BAA-847^T (= CCUG 18818^T). From these data, we propose a new species, ‘H. canicola’ sp. nov., for the isolates from dogs. The type strain is PAGU 1410^T (CCUG 33887^T = LMG 29580^T).