Interferon regulatory factor-2 induces megakaryopoiesis in mouse bone marrow hematopoietic cells
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- 作者：Atsuko Masumi ; Isao Hamaguchi ; Madoka Kuramitsu ; Takuo Mizukami ; Kazuya Takizawa ; Haruka Momose ; Seishiro Naito ; Kazunari Yamaguchi
- 关键词：IRF-2 ; Stem cell ; Inflammation ; Megakaryopoiesis ; Gene regulation
- 刊名：FEBS Letters
- 出版年：2009
- 出版时间：3 November 2009
- 年：2009
- 卷：583
- 期：21
- 页码：3493-3500
- 全文大小：1747 K
文摘
Megakaryopoiesis is associated with inflammatory reactions. To investigate the role of interferon regulatory factors (IRFs) in inflammation-associated megakaryopoiesis, mouse bone marrow hematopoietic stem cells (HSCs) were analyzed. IFN-γ treatment induced IRF-2 expression as well as the expression of CD41 and IRF-1 in HSCs. An in vitro clonogenic assay showed that IRF-2- but not IRF-1-overexpressing cells increased the number of megakaryocytic colonies. IRF-2 transfection up-regulated CD41 promoter activity in hematopoietic cell lines. The number of CD41-positive bone marrow cells increased in mice injected with IRF-2-expressing bone marrow cells. These findings suggest that IRF-2 plays an important role in megakaryopoiesis in inflammatory states.