Species- and concentration-dependent differences of acetyl- and butyrylcholinesterase sensitivity to physostigmine and neostigmine
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- 作者：Diane I. Bitzinger ; diane.bitzinger@ukr.de" class="auth_mail" title="E-mail the corresponding author ; Michael Gruber ; Simon Tü ; mmler ; Bernhard Michels ; Anika Bundscherer ; Susanne Hopf ; Benedikt Trabold ; Bernhard M. Graf ; York A. Zausig
- 关键词：Butyrylcholinesterase ; Acetylcholinesterase ; Alzheimer's disease ; Physostigmine ; Neostigmine ; Inflammatory disease ; Rat ; Ellman's reagent ; Cholinesterase inhibitors
- 刊名：Neuropharmacology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：109
- 期：Complete
- 页码：1-6
- 全文大小：675 K
文摘
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AChE and BChE from human and rat showed marked speciesspecific differences in sensitivity to physostigmine and neostigmine.
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Human AChE and BChE were found to be inhibited in a concentration dependent manner by physostigmine or neostigmine.
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Neostigmine, but not physostigmine, showed a high selectivity for human AChE.
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The inhibition curve of rat BChE showed no concentration-dependency for physostigmine or neostigmine.
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The enzymatic assay enables a rapid and precise determination of ChE-activities in whole blood without sample pretreatment.