On the measurement of consistent long-term retention factor values in micellar liquid chromatography

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• 作者：José ; Marí ; a Bermú ; dez-Saldañ ; a ; Laura Escuder-Gilabert ; Rosa Marí ; a Villanueva-Camañ ; as ; Marí ; a José ; Medina-Herná ; ndez ; Salvador Sagrado
• 关键词：Retention factor estimation ; Liquid chromatography ; Reference retention factors ; Intermediate precision ; Uncertainty
• 刊名：Analytica Chimica Acta
• 出版年：2007
• 出版时间：9 July 2007
• 年：2007
• 卷：595
• 期：1-2
• 页码：19-27
• 全文大小：556 K

文摘

In the field of the quantitative structure–retention and retention–activity relationships (QRAR and QSRR) is crucial to obtain consistent retention factors (k). For this purpose, two unbiased approaches to estimate k are used: (i) the IUPAC approach (based on the extra-column time correction) and (ii) the ‘2-references’ approach (based on the k estimation respect to two prefixed reference k values). Three reference chemicals were selected attending to their retention time, chemical stability and non-ionic character. Consistent retention factor values for these references were estimated for C₁₈ chromatographic columns and Brij35 solutions as mobile phases after statistical analysis. The comparison between the k values estimated for a set of 65 test chemicals using the IUPAC and the ‘2-references’ approach shows that the last one gives consistent long-term values useful for QRAR and QSRR modelling purposes.