The QRAR model study of β-lactam antibiotics by capillary coated with cell membrane

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• 作者：Gengliang Yang ; Weimin Cao ; Tao Zhu ; Ligai Bai ; Yu Zhao
• 关键词：Cell membrane ; Capillary ; β ; -Lactam antibiotics ; QRAR
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2008
• 出版时间：15 September 2008
• 年：2008
• 卷：873
• 期：1
• 页码：1-7
• 全文大小：466 K

文摘

With the accelerating development of new drugs, there is a great need for rapid and simple screening technologies. In this paper, a new in vitro method, capillary coated with cell membrane, was presented for drug screening based on the real biomembrane–drugs interaction, in which the cell membrane was applied to chromatography as pseudo-stationary phase directly. As the cell membrane was coated on the bare-fused capillary via sol–gel technology in our present work, it will be shown to be superior to other pseudo-stationary phases mimicking biological environment. Meanwhile, the quantitative retention–activity relationships (QRAR) model of β-lactam antibiotics was studied through investigating the effect of the membrane coating amount and pH of running buffer on the retention behaviors of the drugs and the logarithm of octanol–water partition coefficient (log P) at pH 7.4 and pH 6.5 were also obtained for comparison. The results showed that the capillary coated with cell membrane could suit the study of QRAR model.