DNA repair in human fibroblasts, as reflected by host-cell reactivation of a transfected UV-irradiated luciferase gene, is not related to donor age
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- 作者：Merkle ; Thomas J. ; O’ ; Brien ; Katherine ; Brooks ; Philip J. ; Tarone ; Robert E. ; Robbins ; Jay H.
- 关键词：cat ; chloramphenical acetyltransferase ; DMEM ; Dulbecco’ ; s modified Eagle’ ; s medium ; FBS ; fetal bovine serum ; HCR ; host-cell reactivation ; luc ; luciferase ; LA ; Luc activity ; NER ; nucleotide excision repair ; pCMV ; pCMVGL3PA ; PDL ; population doubling
- 刊名：Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
- 出版年：2004
- 出版时间：October 4, 2004
- 年：2004
- 卷：554
- 期：1-2
- 页码：9-17
- 全文大小：116 K
文摘
The effect of donor age on the ability of mammalian cells to repair ultraviolet (UV)-induced DNA damage has been studied using several approaches, most recently via assays that measure the host-cell reactivation (HCR) of UV-irradiated reporter gene-containing plasmid vectors following their transfection into cells. Plasmid HCR assays indirectly quantify a cell line’s ability to perform nucleotide excision repair (NER) by measuring the enzyme activity of the repaired reporter gene, e.g., chloramphenical acetyltransferase (cat) or luciferase (luc), and are useful in studies investigating whether increasing age may be a risk factor for the deficient repair of potentially cancer-causing, sunlight-induced, DNA lesions in skin cells. In our study, we quantified the DNA repair ability of cultured, nontransformed, human skin fibroblast lines through their HCR of a transfected UV-C-irradiated plasmid containing luc. HCR was measured at various times after transfection in five lines from normal donors of ages 21–96 years, and from one donor who had xeroderma pigmentosum (XP). The normal lines displayed increasing HCR at successive post-transfection time points and showed no significant correlation between HCR and donor age. The XP-A line, known to be markedly deficient in NER of UV-induced DNA damage, showed minimal evidence of HCR compared to the normal lines. To further assess potential variation in HCR with donor age, fibroblast lines from five old donors, ages 84–94 years, were compared with lines from five young donors, ages 17–26 years. While significant differences in HCR were found between some lines, no significant difference was found between the young and old age groups (P=0.44). Our study provides no indication that the higher incidence of skin cancer observed with increasing age is due to an age-related decrease in the ability to repair UV-induced DNA damage.