Interaction of specifically chemically modified dextrans with transforming growth factor β1: potentiation of its biological activity

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• 作者：Logeart-Avramoglou ; Delphine ; Huynh ; Ré ; mi ; Chaubet ; Fré ; dé ; ric ; Sedel ; Laurent ; et. al.
• 关键词：DCM ; carboxymethyl dextran ; DCMB ; carboxymethyl-benzylamide-dextran ; DCMBSu ; carboxymethyl-benzylamide-sulfate-dextran ; DCMSu ; carboxymethyl-sulfate-dextran ; DMEM ; Dulbecco&#146 ; s modified Eagle&#146 ; s medium ; FCS ; fetal calf serum ; MLEC ; mink lung epithe
• 刊名：Biochemical Pharmacology
• 出版年：2002
• 出版时间：January 15, 2002
• 年：2002
• 卷：63
• 期：2
• 页码：129-137
• 全文大小：223 K

文摘

Transforming growth factor β (TGFβ), a potent multifunctional cytokine, is well known to demonstrate heparin binding ability. This study investigated the binding capacity of heparin-like family of chemically modified dextrans to TGFβ1. Dextran derivatives with various substitution contents in carboxymethyl, benzylamide and sulfate groups were evaluated using a gel mobility shift assay. This structure–function study indicated that a synergistic role of benzylamide and sulfate substituents resulted in an optimal interaction with the growth factor. The effect of these polymers on the biological response of TGFβ1 was assessed using mink lung epithelial cells transfected with a plasminogen activator inhibitor-1 promoter-luciferase construct (PAI/Luc). When the growth factor was mixed with 250μg/mL of carboxymethyl-benzylamide-dextran (DCMB) or carboxymethyl-benzylamide-sulfate-dextran (DCMBSu), the luciferase gene expression was enhanced. Only polymers exhibiting TGFβ1 binding demonstrated a biological potentiating effect. However, this effect was strongly amplified as the cell plating time increased (35-fold increase with a 2 days plating time versus 1.1-fold increase with a 4hr plating time at a 0.25ng/mL concentration of TGFβ1). TGFβ1 induced the PAI/Luc construct in a dose-dependent fashion but its effect diminished when added to cells previously cultured for 24 and 48hr. The results indicated that the potentiating effect required a complex formation between TGFβ1 and polymers, the action of which seeming to locally maintain TGFβ1 in an active form. TGFβ isoforms playing a key role in the process of bone repair, specifically designed functionalized dextrans could potentiate the in vivo TGFβ1 biological effect and be used in the field of wound healing applications.