Amiodarone biokinetics, the formation of its major oxidative metabolite and neurotoxicity after acute and repeated exposure of brain cell cultures
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- 作者：Giuliana Pomponio<sup>asup><sup> ; sup><sup>dsup><sup> ; sup><sup>1sup> ; Marie-Gabrielle Zurich<sup>bsup><sup> ; sup><sup>1sup> ; Luise Schultz<sup>csup><sup> ; sup><sup>1sup> ; Dieter G. Weiss<sup>csup> ; Luca Romanelli<sup>dsup> ; Alexandra Gramowski-Voss<sup>csup> ; Emma Di Consiglio<sup>asup><sup> ; sup><sup>emma.diconsiglio@iss.it" class="auth_mail" title="E-mail the corresponding authorsup> ; Emanuela Testai<sup>asup>
- 关键词：AMI ; Amiodarone ; MDEA ; mono-N-desethylamiodarone ; DDEA ; di-N-desethylamiodarone ; 2D mouse model ; murine neuronal network culture in two dimensional structure ; 3D rat model ; re-aggregating rat brain cell cultures in three dimensional structure ; DIV ; days in vitro ; LDH ; lactate dehydrogenase ; CYP ; cytochrome P450 ; DMSO ; Dimethylsulfoxide ; DMEM ; Dulbecco&rsquo ; s Modified Eagle&rsquo ; s medium ; PBS ; phosphate buffered saline ; PNGM ; Primary Neuron Growth Medium ; PNBM ; Primary Neuron Basal Medium ; d0
- 刊名：Toxicology in Vitro
- 出版年：2015
- 出版时间：25 December 2015
- 年：2015
- 卷：30
- 期：1
- 页码：192-202
- 全文大小：641 K
文摘
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Amiodarone biokinetics was studied in 2D and 3D brain cell models.
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After single treatment Amiodarone cell uptake was completed after 24 h.
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After repeated exposure Amiodarone bioaccumulates, especially in the 3D cell model.
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Bioaccumulation is paralleled to some alterations of neurotoxicity markers.
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The two brain cell models are able to form the Amiodarone major oxidative metabolite.