Amiodarone biokinetics was studied in 2D and 3D brain cell models.
After single treatment Amiodarone cell uptake was completed after 24 h.
After repeated exposure Amiodarone bioaccumulates, especially in the 3D cell model.
Bioaccumulation is paralleled to some alterations of neurotoxicity markers.
The two brain cell models are able to form the Amiodarone major oxidative metabolite.