Proteomic investigation of embryonic rat heart-derived H9c2 cell line sheds new light on the molecular phenotype of the popular cell model
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- 作者：Juraj Lenčo<sup>asup> ; Olga Lenčová ; -Popelová ; <sup>bsup> ; Marek Link<sup>asup> ; Anna Jirkovská ; <sup>csup> ; Vojtěch Tambor<sup>dsup> ; Eli&scaron ; ka Potůčková ; <sup>csup> ; Jiří ; Stulí ; k<sup>asup> ; Tomá ; &scaron ; &Scaron ; imůnek<sup>csup> ; Martin &Scaron ; těrba<sup>bsup><sup> ; sup><sup>sterbam@lfhk.cuni.cz" class="auth_mail" title="E-mail the corresponding authorsup>
- 关键词：AcN ; acetonitrile ; aGPC ; adult guinea pig cardiomyocytes ; aLVM ; adult rat left ventricular myocardium ; ATRA ; all-trans retinoic acid ; CTGF ; connective tissue growth factor ; DMEM ; Dulbecco´ ; s modified Eagle´ ; s medium ; FBS ; fetal bovine serum ; GAPDH ; glyceraldehyde-3-phosphate dehydrogenase ; H-FABP ; heart fatty acid-binding protein ; LC-MALDI ; liquid chromatography-matrix assisted laser desorption/ionization ; LC-SRM ; liquid chromatography-selected reaction monitoring ; nVCM ; neonatal ventr
- 刊名：Experimental Cell Research
- 出版年：2015
- 出版时间：10 December 2015
- 年：2015
- 卷：339
- 期：2
- 页码：174-186
- 全文大小：1546 K
文摘
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Proteomics was used to characterize phenotype of undifferentiated H9c2 cells.
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Heart-derived H9c2 cells were found considerably different from cardiac myocytes.
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Doxorubicin does not induce typical cardiotoxic proteomic signature in H9c2 cells.
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Interpretation of findings obtained on H9c2 cells deserves caution and verification.