Gallic acid reduces cell growth by induction of apoptosis and reduction of IL-8 in HepG2 cells
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- 作者：Kelly Goulart Lima<sup>asup><sup> ; sup><sup>kelly.lima@acad.pucrs.brsup> ; Gabriele Catyana Krause<sup>asup> ; Aline Daniele Schuster<sup>bsup> ; Anderson Velasque Catarina<sup>csup> ; Bruno Souza Basso<sup>asup> ; Fernanda Cristina De Mesquita<sup>asup> ; Leonardo Pedrazza<sup>asup> ; Elisa Simon Marczak<sup>dsup> ; Bianca Andrade Martha<sup>asup> ; Fernanda Bordignon Nunes<sup>asup> ; Eduardo Cremonese Filippi Chiela<sup>esup> ; Natá ; lia Jaeger<sup>dsup> ; Marcos Paulo Thomé ; <sup>fsup> ; Gabriela Viegas Haute<sup>asup> ; Henrique Bregolin Dias<sup>asup> ; Má ; rcio Viní ; cius Fagundes Donadio<sup>gsup> ; Jarbas Rodrigues De Oliveira<sup>asup>
- 关键词：7-AAD ; 7-Amino-Actinomycin D ; ANOVA ; one-way analysis of variance ; BrdU ; Bromodeoxyuridine ; CC50 ; 50% cytotoxic concentration ; CPD ; Cumulative population doubling ; DAPI ; 40 ; 6-diamidino-2-phenylindole ; DMEM ; Dulbecco&rsquo ; s modified Eagle medium ; FBS ; fetal bovine serum ; FITC ; Fluorescein ; GA ; Gallic acid ; HCC ; Hepatocelullar carcinoma ; HEPES ; 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid ; HepG2 ; liver carcinoma cells ; IL-10 ; Interleukin 10 ; IL-12 ; Interleukin 12 ; IL-1β ; Interleukin 1β ; IL-
- 刊名：Biomedicine & Pharmacotherapy
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：84
- 期：Complete
- 页码：1282-1290
- 全文大小：2014 K
- 卷排序：84
文摘
Hepatocellular carcinoma is the most prevalent primary liver tumor and is among the top ten cancer that affect the world population. Its development is related, in most cases, to the existence of chronic liver injury, such as in cirrhosis. The knowledge about the correlation between chronic inflammation and cancer has driven new researches with anti-inflammatory agents that have potential for the development of antitumor drugs. Gallic acid is a phenolic acid found in many natural products and have shown anti-inflammatory, anti-tumor, anti-mutagenic and antioxidant actions. The purpose of this study was to investigate the effect of gallic acid on acute and chronic cell proliferation and inflammatory parameters of hepatocellular carcinoma cells (HepG2), as well as to investigate the mechanisms involved. Results showed that the gallic acid decreased the proliferation of HepG2 cells in a dose-dependent manner (Trypan blue exclusion assay), without causing necrosis (LDH assay). We observed a significant increase in the percentage of small and regular nuclei (Nuclear Morphometric Analysis assay), a significant induction of apoptosis by Annexin V-FITC and PI assay and no interference with the cell cycle using the FITC BrdU Flow Kit. We observed a significant reduction in the levels of IL-8 and increased levels of IL-10 and IL-12 (Cytometric Bead Array Human Inflammation Assay). Furthermore, gallic acid caused no cancer cells regrowth at a long term (Cumulative Population Doubling assay). According to these results, gallic acid showed a strong potential as an anti-tumor agent in hepatocellular carcinoma cells.