3D Cryo-Electron Reconstruction of BmrA, a Bacterial Multidrug ABC Transporter in an Inward-Facing Conformation and in a Lipidic Environment
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- 作者：Pierre Frederic Fribourg1 ; 2 ; canapif@gmail.com" class="auth_mail ; Mohamed Chami3 ; mohamed.chami@unibas.ch" class="auth_mail ; Carlos Oscar S. Sorzano4 ; coss@cnb.csic.es" class="auth_mail ; Francesca Gubellini1 ; 2 ; 1 ; francesca.gubellini@pasteur.fr" class="auth_mail ; Roberto Marabini4 ; roberto.marabini@uam.es" class="auth_mail ; Sergio Marco1 ; 5 ; Sergio.marco@curie.fr" class="auth_mail ; Jean-Michel Jault6 ; 2 ; jean-michel.jault@ibcp.fr" class="auth_mail ; Daniel Lé ; vy1 ; 2 ; 7 ; daniel.levy@curie.fr" class="auth_mail
- 关键词：DDM ; N-dodecyl-尾-d-maltoside ; EPC ; egg phosphatidylcholine ; EPA ; egg phosphatidic acid ; DOPC ; dioleoyl-phosphatidylcholine ; cryo-EM ; cryo-electron microscopy ; 2D ; two-dimensional ; 3D ; three-dimensional ; TMD ; transmembrane domain ; NBD ; nucleotide-binding domain ; ICD ; intracellular domain ; P-gp ; P-glycoprotein ; LRET ; luminescence resonance energy transfer ; DEER ; double electron&ndash ; electron resonance ; FRET ; fluorescence resonance energy transfer
- 刊名：Journal of Molecular Biology
- 出版年：15 May 2014
- 年：2014
- 卷：426
- 期：10
- 页码：2059-2069
- 全文大小：1458 K
文摘
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The apo-conformation of ABC transporters is a key conformation of drugs transport.
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A three-dimensional model from cryo-electron microscopy of the apo-conformation of BmrA in lipid membrane is described.
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Two open inward conformations of BmrA with disengaged NBDs are coexisting.
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The open outward conformation is derived from two-dimensional crystals.
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ABC transporters show a large flexibility in the apostate in lipid membrane.