Integrating insulin into single-step culture medium regulates human embryo development in vitro

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• 作者：Mohamed Fawzy ; M.D.^a ; ^{drfawzy001@me.com} ; Mohamed Sabry ; M.D.^b ; Mohamed Nour ; M.D.^b ; Mohamed Y. Abdelrahman ; M.D.^b ; Eman Roshdy ; M.D.^c ; Yasmin Magdi ; M.Sc.^d ; Hazem Abdelghafar ; M.D.^b
• 关键词：Embryo culture media ; insulin embryonic effect ; blastocyst
• 刊名：Fertility and Sterility
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：107
• 期：2
• 页码：405-412
• 全文大小：264 K
• 卷排序：107

文摘

To evaluate the effect of supplementing single-step embryo culture medium with insulin on human embryo development.DesignComparative study.SettingTwo private centers.Patient(s)The study involved a sibling oocyte split of 5,142 retrieved oocytes from 360 patients.Intervention(s)Sibling oocytes split after intracytoplasmic sperm injection for culture from day 0 through day 5 or 6 in insulin-supplemented or control medium. Women were split to receive their embryos from insulin-supplemented or control medium.Main Outcome Measure(s)Clinical pregnancy rate.Result(s)There were significantly higher rates of clinical, ongoing, and twin pregnancies in the insulin-supplemented arm than in the control arm. On day 3, embryo quality and compaction were higher in insulin-supplemented medium. On day 5, insulin supplementation showed higher rates of blastocyst formation, quality, and cryopreservation.Conclusion(s)Insulin supplementation of single-step embryo culture medium from day 0 through day 5 or 6 improved clinical pregnancy rate and human embryo development. However, these findings need further confirmation through a multicenter randomized controlled trial that may include other patient populations and different culture media.