Association between Interleukin-10 -1082G/A Gene Polymorphism and Risk of Stroke in the North Indian Population: A Case-Control Study

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• 作者：Pradeep Kumar ; PhD^* ; Amit Kumar ; PhD^* ; Ram Sagar ; MSc^* ; Shubham Misra ; M.Tech^* ; Mohammad Faruq ; PhD^&dagger ; ; Varun Suroliya ; MSc^* ; Subiah Vivekanandhan ; PhD^&Dagger ; ; Achal Kumar Srivastava ; DM^* ; Kameshwar Prasad ; DM^* ; ^{kp0704@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ischemic stroke ; intracerebral hemorrhage ; cerebrovascular disease ; single-nucleotide polymorphism ; promoter ; interleukin-10 ; anti-inflammatory cytokine
• 刊名：Journal of Stroke and Cerebrovascular Diseases
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：25
• 期：2
• 页码：461-468
• 全文大小：130 K

文摘

Anti-inflammatory interleukin-10 (IL-10) cytokine and its genetic variations may play an important role in the pathogenesis of various human diseases including stroke.

Objective

The aim of this present case–control study was to determine the association between IL-10 -1082G/A (rs1800896) gene polymorphism and risk of stroke in the North Indian population.

Methods

Genotyping was carried out by using SNaPshot method (Applied Biosystems, Foster City, California, United States) for 250 ischemic stroke (IS) patients, 250 age- and sex-matched IS free controls, 100 intracerebral hemorrhage (ICH) patients, and 100 age- and sex-matched ICH free controls. IS was classified using the Trial of Org 10172 in Acute Stroke Treatment classification. Conditional logistic regression analysis with adjustment for multiple demographic and risk factor variables was used to calculate the strength of association between IL-10 (-1082G/A) polymorphism and risk of stroke.

Results

Conditional logistic regression analysis showed an independent association between IL-10 -1082G/A and risk of IS under a dominant model (odds ratio [OR] = 2.39, 95% confidence interval [CI] = 1.34-4.27, P = .003) and an allelic model (OR = 2.49, 95% CI 1.71-3.63, P < .001). An independent association between IL-10 -1082G/A, under the dominant model (OR = 6.8, 95% CI 2.2-20.7, P < .001) and the allelic model (OR = 3.4, 95% CI 1.8-6.3, P < .001), and the risk of ICH was also observed.

Conclusion

Our results suggest that IL-10 -1082G/A gene polymorphism is an independent risk factor for the risk of IS and ICH in the North Indian population. Our findings indicate that IL-10 -1082G/A polymorphism may be used as a genetic marker for identifying individuals at increased risk of developing stroke.