Live cell monitoring of glycine betaine by FRET-based genetically encoded nanosensor
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- 作者：Mohammad Ahmada ; Seema Ameena ; Tariq Omar Siddiqia ; Parvez Khanb ; Altaf Ahmadc ; aahmad.bo@amu.ac.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：Compatible solute ; Salinity ; Glycine betaine ; Fluorescence resonance energy transfer ; Green fluorescent protein
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：169-175
- 全文大小：1506 K
文摘
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We developed a FRET-based genetically encoded nanosensor for the detection of glycine betaine.
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This sensor allows the non-destructive real-time monitoring of glycine betaine in cells.
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The monitoring of glycine betaine can be performed in prokaryotic as well eukaryotic cell.
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This nanosensor would help to increase quantitative knowledge of fluctuations of GB level in living cells.