Optimal compressive force induces bone formation via increasing bone sialoprotein and prostaglandin E₂ production appropriately

详细信息    查看全文

• 作者：Narihiro Mitsui ; Naoto Suzuki ; Masao Maeno ; Kotoe Mayahara ; Momoko Yanagisawa ; Kichibee Otsuka and Noriyoshi Shimizu
• 关键词：Mechanical stress ; Osteoblasts ; Mineralization ; Bone sialoprotein ; Prostaglandin E₂
• 刊名：Life Sciences
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：77
• 期：25
• 页码：3168-3182
• 全文大小：612 K

文摘

Although orthodontic tooth movement can promote bone formation, the molecular mechanism that underlies this phenomenon is not fully understood. The purposes of this study were to determine how mechanical stress affects the osteogenic response of human osteoblastic cells (Saos-2), and also examine the optimal compression for osteogenesis in vitro. Saos-2 cells cultured with or without continuously compressive force (0.53.0 g/cm²). The expression of bone sialoprotein (BSP), osteopontin, and cyclooxygenase-2 (COX-2) were measured using real-time PCR, Western blot analysis and immunoassay. The calcium content in the mineralized nodules was determined using Calcium C-Test kit. Only one loading with 1.0 g/cm² of compressive force significantly increased the expression of BSP mRNA and protein, COX-2 mRNA expression and PGE₂ synthesis. Indomethacin, an inhibitor of PGE₂ synthesis, inhibited the compression-induced above phenomenon. Moreover, the conditioned medium from 1.0 g/cm² of compressive force apparently stimulated calcium content in mineralized nodules. This study demonstrates that an optimal compressive force stimulates in vitro mineralization by BSP synthesis through the autocrin action of PGE₂ production.