Compressive force stimulates the expression of osteogenesis-related transcription factors in ROS 17/2.8 cells

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• 作者：Momoko Yanagisawa ; Naoto Suzuki ; Narihiro Mitsui ; Yuki Koyama ; Kichibee Otsuka ; Noriyoshi Shimizu
• 关键词：Mechanical stress ; Osteogenesis ; ROS 17/2.8 ; Transcription factors
• 刊名：Archives of Oral Biology
• 出版年：2008
• 出版时间：March 2008
• 年：2008
• 卷：53
• 期：3
• 页码：214-219
• 全文大小：483 K

文摘

To determine how compressive force affects the expression of osteogenesis-related transcription factors in osteoblasts.

Design

Cells of ROS 17/2.8, a typical osteoblastic cell line, were cultured with or without continuous compressive force (0.5–2.0 g/cm²). Expression of mRNA encoding the osteogenesis-related transcription factors Runx2, Osterix, Msx2, Dlx5 and AJ18 was measured using real-time polymerase chain reaction. Protein expression of these transcription factors was determined by Western blotting.

Results

A compressive force of 1.0 g/cm² significantly increased mRNA and protein expression of Runx2, Osterix, Msx2 and Dlx5, which are critical for osteoblast differentiation. In contrast, mRNA and protein expression of AJ18, which downregulates osteoblast differentiation, were decreased with 1.0 g/cm² of compressive force.

Conclusions

A compressive force of 1.0 g/cm², which was considered optimal for bone formation under the present experimental conditions, stimulates osteoblastic differentiation via the modulation of osteogenesis-related transcription factors.