A rapid profiling of hypolipidemic agents in dietary supplements by direct injection tandem mass spectrometry

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• 作者：Miranda Serti膰 ; Ana Mornar ; ^{amornar@pharma.hr" class="auth_mail} ; Biljana Nigovi膰
• 关键词：Natural cholesterol-lowering products ; Dietary supplements safety ; Regulatory challenge ; Supplement composition ; Direct injection mass spectrometry ; Fingerprint analysis ; Fragment analysis ; Hyperlipidemia ; Food composition ; Food analysis ; Food safety
• 刊名：Journal of Food Composition and Analysis
• 出版年：May, 2014
• 年：2014
• 卷：34
• 期：1
• 页码：68-74
• 全文大小：700 K

文摘

A new, fast-screening method for identification of various pharmacologically active ingredients in cholesterol-lowering dietary supplements using direct injection mass spectrometry was developed. The optimized method was proven to be useful for multitarget screening: 17 compounds of interest were identified, including monacolin K and its acid form, cynarin, green tea catechines, genistein, daidzein, guggulsterones, allicin and l-arginine. The method was successfully applied for the fingerprint analysis of dietary supplements containing only one or several cholesterol-lowering agents including lovastatin registered as a drug. According to the obtained results, it is apparent that cholesterol-lowering dietary supplements are poorly standardized, as two samples did not contain active ingredients (monacolin K, monacolin K acid form and cynarin). Moreover, the application of the proposed direct injection method to quality control of cholesterol-lowering dietary supplements may be proposed for the routine analysis of a large number of samples in the laboratories of regulatory agencies.