Regulation of insulin-like growth factor-I and thioredoxin expression by estradiol in the reproductive tract of the prepubertal female lamb

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• 作者：Sahlin ; Lena ; Meikle ; Ana ; Tasende ; Celia ; Lindberg ; Monica ; Masironi ; Britt ; Eriksson ; Hå ; kan
• 关键词：IGF-I ; Thioredoxin ; Cervix ; Uterus ; Oviduct
• 刊名：The Journal of Steroid Biochemistry and Molecular Biology
• 出版年：2001
• 出版时间：May, 2001
• 年：2001
• 卷：77
• 期：2-3
• 页码：123-128
• 全文大小：135 K

文摘

Estradiol (E₂) has been shown to be an important uterine growth promoting molecule in the ovariectomized (ovx) rat, which increases the mRNA levels of insulin-like growth factor-I (IGF-I) and the redox enzyme thioredoxin. The aim of this study was to explore the role of E₂ in the regulation of IGF-I and thioredoxin in the reproductive tract of the prepubertal female lamb. Twenty 3-month-old lambs were treated with i.m. injections of E₂ at 24 h intervals. The animals were sacrificed 12 or 24 h after the last injection, and 72 h was the longest treatment period. The mRNA levels of thioredoxin and IGF-I were determined by a solution hybridization technique. There was a 5-fold increase in the cervical IGF-I mRNA level 12 h after the first E₂ injection. The uterine IGF-I mRNA level was doubled after 12 h and this increase was maintained during the rest of the experimental period. The IGF-I mRNA level in the oviducts was more than doubled 12 and 24 h after the E₂ injection, then the level decreased towards the initial level. The thioredoxin mRNA level in the cervix was increased 4-fold after 24 h, whereas no significant effect was seen in the uterus. The thioredoxin mRNA level in the oviduct was more than doubled 12 and 24 h after the first E₂ injection. Thus, estradiol regulates the expression of IGF-I and thioredoxin in the reproductive tract of prepubertal lambs.