Remodeling of tissue-engineered bone structures in vivo

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• 作者：S ; ra Hofmann ; Monika Hilbe ; Robert J. Fajardo ; Henri Hagenm¨¹ller ; Katja Nuss ; Margarete Arras ; Ralph M¨¹ller ; Brigitte von Rechenberg ; David L. Kaplan ; Hans P. Merkle ; Lorenz Meinel
• 关键词：Remodeling ; Tissue engineering ; Bone ; In vivo ; Stem cell ; Silk fibroin
• 刊名：European Journal of Pharmaceutics and Biopharmaceutics
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：85
• 期：1
• 页码：119-129
• 全文大小：4625 K

文摘

Implant design for bone regeneration is expected to be optimized when implant structures resemble the anatomical situation of the defect site. We tested the validity of this hypothesis by exploring the feasibility of generating different in vitro engineered bone-like structures originating from porous silk fibroin scaffolds decorated with RGD sequences (SF-RGD), seeded with human mesenchymal stem cells (hMSC). Scaffolds with small (106-212 ¦Ìm), medium (212-300 ¦Ìm), and large pore diameter ranges (300-425 ¦Ìm) were seeded with hMSC and subsequently differentiated in vitro into bone-like tissue resembling initial scaffold geometries and featuring bone-like structures. Eight weeks after implantation into calvarial defects in mice, the in vitro engineered bone-like tissues had remodeled into bone featuring different proportions of woven/lamellar bone bridging the defects. Regardless of pore diameter, all implants integrated well, vascularization was advanced, and bone marrow ingrowth had started. Ultimately, in this defect model, the geometry of the in vitro generated tissue-engineered bone structure, trabecular- or plate-like, had no significant impact on the healing of the defect, owing to an efficient remodeling of its structure after implantation.