Taurine ameliorate alloxan induced oxidative stress and intrinsic apoptotic pathway in the hepatic tissue of diabetic rats
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- 作者：Kahkashan Rashid ; Joydeep Das ; Parames C. Sil ; parames@bosemain.boseinst.ac.in
- 关键词：ALP ; alkaline phosphatase ; ALT ; alanine transaminase ; ALX ; alloxan monohydrate ; ATP ; adenosine tri phosphate ; CAT ; catalase ; DAB ; 3 ; 3&prime ; -diaminobenzidine tetrahydrochloride ; GLUT 2 ; glucose transporter type 2 ; GPx ; glutathione peroxidase ; GR ; glutathi
- 刊名：Food and Chemical Toxicology
- 出版年：2013
- 出版时间：January, 2013
- 年：2013
- 卷：51
- 期：Complete
- 页码：317-329
- 全文大小：1342 K
文摘
Oxidative stress is associated with various diabetic complications and taurine plays an important role in ameliorating those difficulties. In the present study we, therefore, investigated whether taurine plays any beneficial role against diabetes induced liver dysfunction and if it does, what cellular mechanism it follows during protective action. Induction of diabetes by alloxan (ALX) (at a dose of 120 mg/kg body weight, i.p., once) reduced body weight and plasma insulin level, enhanced blood glucose and serum markers related to hepatic injury, accelerated ROS production, disturbed the intra-cellular antioxidant machineries and disintegrated hepatic cells near central vein. This pathophysiology leads to apoptotic cell death as evidenced from DNA fragmentation and TUNEL aasay. Studies on the mechanism of apoptosis showed that ALX accelerated the markers of mitochondrial dependent apoptotic pathway (enhanced cytochrome C release in cytosol from mitochondria, altered the expression of Bax, Bcl-2, Apaf-1, caspase-9, caspase-3). Treatment with taurine (1 % w/v for three weeks) post-hyperglycemia, however, could restore all the alteration caused by ALX. Moreover, taurine activates hepatic PI3Kinase, Akt, hexokinase and augments the translocation of GLUT 2 to hepatic membrane in diabetic rats. Combining all, as a potential therapeutic, taurine may normalize the complications of diabetic liver injury.