HDAC inhibitors induce global changes in histone lysine and arginine methylation and alter expression of lysine demethylases
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- 作者：Ryan Lillico ; Marina Gomez Sobral ; Nicholas Stesco ; Ted M. Lakowski ; ted.lakowski@umanitoba.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：HDAC ; histone deacetylase ; HAT ; histone acetyltransferase ; HKMT ; histone lysine methyltransferase ; KDM ; lysine demethylase ; H3K4Me ; histone 3 monomethyllysine 4 ; H3K4Me2 ; histone 3 dimethyllysine 4 ; H3K4Me3 ; histone 3 trimethyllysine 4 ; H3K4Ac ; histone 3 acetyllysine 4 ; AcK ; acetyllysine ; MeK ; monomethyllysine ; Me2K ; dimethyllysine ; Me3K ; trimethyllysine ; MeR ; monomethylarginine ; aMe2R ; asymmetric dimethylarginine ; sMe2R ; symmetric dimethylarginine ; LC&ndash ; MS/MS ; liquid chromatography tandem m
- 刊名：Journal of Proteomics
- 出版年：2016
- 出版时间：5 February 2016
- 年：2016
- 卷：133
- 期：Complete
- 页码：125-133
- 全文大小：1175 K
文摘
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We develop an LC–MS/MS method measuring epigenetic modifications from cells.
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HDAC increases histone lysine acetylation correlating with cell viability by MTS.
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The effects on histone methylation and KDM expression depend on the HDAC inhibitor.
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Mocetinostat increases histone lysine methylation and decreases expression of KDM.
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Mocetinostat is both an HDAC inhibitor and a de facto KDM inhibitor.