Detection of N-monomethyllysine using high-performance liquid chromatography and high-performance liquid chromatography–mass spectrometry

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• 作者：Kal??sz ; H. ; Sz??cs ; Z. ; Tihanyi ; M. ; Szil??gyi ; ??. ; Lengyel ; J.
• 关键词：l-Deprenyl ; N ^; //www.sciencedirect.com/scidirimg/entities/25b.gif"" alt=""var epsilon"" border=0>-Monomethyllysine ; Transmethylation ; HPLC ; HPLC/MS
• 刊名：Journal of Chromatography A
• 出版年：2005
• 出版时间：June 24, 2005
• 年：2005
• 卷：1079
• 期：1-2
• 页码：208-212
• 全文大小：145 K

文摘

N-Monomethyllysine was identified in the serum, urine, brain, and liver samples of rats treated per os with l-deprenyl. The identification procedure included reaction with Fmoc chloride, clean-up, and analysis using HPLC–UV–MS. Oral administration of (−)-N-¹⁴C-methyl-N-propynyl(2-phenyl-1-methyl)ethylammonium hydrochloride l-deprenyl) to rats resulted in transfer of the radiolabelled methyl group to the N-amino group of the endogenous lysine. The radiolabelled N-monomethyllysine was urinary eliminated together with the other radiolabelled deprenyl metabolites, such as deprenyl-N-oxide and methamphetamine. The presence of N-monomethyllysine has also been traced, and its concentrations were compared in the serum, liver and brain of rats subjected to l-deprenyl treatment. Methyl group transfer from the l-deprenyl to endogenous compounds; and the urinary elimination of their products may offer a vital way to eliminate or to decrease the degree of drug transmethylation to the lysine constituents of blood vessels’ proteins.