Gene structure and regulation of alkane monooxygenases in propane-utilizing Mycobacterium sp. TY-6 and Pseudonocardia sp. TY-7

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• 作者：Tetsuya  ; Kotani ; Yui  ; Kawashima ; Hiroya  ; Yurimoto ; Nobuo  ; Kato¹ ; Yasuyoshi  ; Sakai  ; ;   ; ^{ysakai@kais.kyoto-u.ac.jp}
• 关键词：propane-utilizing bacteria ; alkane ; alkane monooxygenase system ; propane monooxygenase ; alkane hydroxylase ; propane oxidation pathway
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2006
• 出版时间：September 2006
• 年：2006
• 卷：102
• 期：3
• 页码：184-192
• 全文大小：612 K

文摘

Mycobacterium sp. TY-6 and Pseudonocardia sp. TY-7 were isolated from soil samples as propane-utilizing bacteria and were found to be able to utilize various gaseous and liquid n-alkanes as carbon and energy sources. One gene cluster, M-prmABCD, and two gene clusters, P-prm1ABCD and P-prm2ABCD, were cloned from the genomes of Mycobacterium sp. TY-6 and Pseudonocardia sp. TY-7, respectively. These gene clusters are homologous to the gene cluster encoding the multicomponent propane monooxygenase (prmABCD) of Gordonia sp. TY-5. The expression of prm gene clusters in Mycobacterium sp. TY-6 and Pseudonocardia sp. TY-7 was shown to be induced by gaseous n-alkanes (C₂¨CC₄) except methane, suggesting that the products of these genes are involved in gaseous n-alkane oxidation. Homologous genes for an alkane hydroxylase system (alk system) involved in liquid n-alkane oxidation were also cloned from the genomic DNA of Mycobacterium sp. TY-6. The alk gene cluster was transcribed in response to liquid n-alkanes (C₁₁¨CC₁₅). These results indicate that Mycobacterium sp. TY-6 has two distinct gene clusters for multicomponent monooxygenases involved in alkane oxidation. Whole-cell reactions revealed that propane is oxidized to 1-propanol through terminal oxidation in Mycobacterium sp. TY-6 and that propane is oxidized to 1-propanol and 2-propanol through both terminal and subterminal oxidations in Pseudonocardia sp. TY-7. This study reveals the diversity of propane metabolism present in microorganisms.