Overexpression of erg20 gene encoding farnesyl pyrophosphate synthase has contrasting effects on activity of enzymes of the dolichyl and sterol branches of mevalonate pathway in Trichoderma reesei
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- 作者：Sebastian Pi艂syk ; Urszula Perli艅ska-Lenart ; Wioletta Gó ; rka-Nie膰 ; Sebastian Graczyk ; Beata Antosiewicz ; Patrycja Zembek ; Gra偶yna Palamarczyk ; Joanna S. Kruszewska ; jsk@ibb.waw.pl" class="auth_mail
- 关键词：DMAPP ; dimethylallylpyrophosphate ; erg20 ; gene encoding FPP synthase ; erg9 ; gene encoding squalene synthase ; ERGXX ; FPP synthase protein ; FPP ; farnesyl pyrophosphate ; GPP ; geranyl pyrophosphate ; HMGR ; 3-hydroxy-3-methylglutaryl-CoA reductase ; IPP ; isopentenyl pyrophosphate ; pyr4 ; gene encoding orotidine-5&prime ; -monophosphate decarboxylase
- 刊名：Gene
- 出版年：10 July 2014
- 年：2014
- 卷：544
- 期：2
- 页码：114-122
- 全文大小：1717 K
文摘
- 5">
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5">erg20 gene encoding FPP synthase from T. reesei was cloned and functionally analyzed.
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The gene overexpressed in T. reesei elevated FPP synthase activity
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5">Dolichyl branch of mevalonate pathway was stimulated in the transformants.
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In the erg20 overexpressing strains squalene synthase was inhibited.
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5">Inhibition of squalene synthase did not affect ergosterol level.