A preventive immunization approach against insect bite hypersensitivity: Intralymphatic injection with recombinant allergens in Alum or Alum and monophosphoryl lipid A

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• 作者：Sigridur Jonsdottir^a ; ^{sij9@hi.is" class="auth_mail" title="E-mail the corresponding author} ; Vilhjalmur Svansson^a ; Sara Bjork Stefansdottir^a ; Gertraud Schü ; pbach^b ; Claudio Rhyner^c ; Eliane Marti^b ; ¹ ; Sigurbjorg Torsteinsdottir^a ; ¹
• 关键词：Horses ; Insect bite hypersensitivity ; Immunotherapy ; Adjuvants ; IgG subclasses
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：172
• 期：Complete
• 页码：14-20
• 全文大小：1104 K

文摘

Insect bite hypersensitivity (IBH) is an IgE-mediated dermatitis of horses caused by bites of Culicoides insects, not indigenous to Iceland. Horses born in Iceland and exported to Culicoides-rich areas are frequently affected with IBH.

ar0035">The aims of the study were to compare immunization with recombinant allergens using the adjuvant aluminum hydroxide (Alum) alone or combined with monophosphoryl lipid A (MPLA) for development of a preventive immunization against IBH.

ar0040">Twelve healthy Icelandic horses were vaccinated intralymphatically three times with 10 μg each of four recombinant Culicoides nubeculosus allergens in Alum or in Alum/MPLA.

ar0045">Injection with allergens in both Alum and Alum/MPLA resulted in significant increase in specific IgG subclasses and IgA against all r-allergens with no significant differences between the adjuvant groups. The induced antibodies from both groups could block binding of allergen specific IgE from IBH affected horses to a similar extent. No IgE-mediated reactions were induced. Allergen-stimulated PBMC from Alum/MPLA horses but not from Alum only horses produced significantly more IFNγ and IL-10 than PBMC from non-vaccinated control horses.

ar0050">In conclusion, intralymphatic administration of small amounts of pure allergens in Alum/MPLA induces high IgG antibody levels and Th1/Treg immune response and is a promising approach for immunoprophylaxis and immunotherapy against IBH.