Influence of polar side chains modifications on the dual enkephalinase inhibitory activity and conformation of human opiorphin, a pain perception related peptide

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• 作者：Mò ; nica Rosa^a ; Filipa Marcelo^b ; Luis P. Calle^c ; Catherine Rougeot^d ; Jesú ; s Jimé ; nez-Barbero^c ; ^e ; Gemma Arsequell^a ; Gregorio Valencia^a ;
• 关键词：Opiorphin analogs ; SAR study ; Neutral endopeptidase ; AP-N ; Glycopeptides pain
• 刊名：Bioorganic & Medicinal Chemistry Letters
• 出版年：2015
• 出版时间：15 November 2015
• 年：2015
• 卷：25
• 期：22
• 页码：5190-5193
• 全文大小：537 K

文摘

The dual inhibitory action of the pain related peptide opiorphin (H-Gln-Arg-Phe-Ser-Arg-OH) against neutral endopeptidase (NEP) and aminopeptidase N (AP-N) was further investigated by a SAR study involving minor modifications on the polar side chains of Arg residues and glycosylation with monosaccharides at Ser. None of them exerted dual or individual inhibitory potency superior than opiorphin. However, the correlations deduced offer further proof for the key role of these residues upon the binding and bioactive conformational stabilization of opiorphin. NMR conformational studies on the glycopeptides suggest that they are still very flexible compounds that may attain their respective bioactive conformations.