Structure elucidation and immunomodulatory activity in vitro of a xylan from roots of Cudrania tricuspidata

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• 作者：Lei Shi ; Qun Dong ; Kan Ding
• 关键词：Cudrania tricuspidata ; Xylan ; Structure ; Immunomodulatory activity ; Purification
• 刊名：Food Chemistry
• 出版年：1 June, 2014
• 年：2014
• 卷：152
• 期：Complete
• 页码：291-296
• 全文大小：708 K

文摘

To discover new immunomodulator in the food industry, a water-soluble polysaccharide, CTPB1 was obtained from roots of Cudrania tricuspidata (Carr.) Bur. The average molecular weight of this polysaccharide was estimated to be 4.0 脳 10⁴ Da. Monosaccharide composition analysis indicated that the only neutral sugar present in this polysaccharide was xylose. From methylation analysis and ¹H and ¹³C NMR spectroscopy, the structure of CTPB1 was deduced to be (4-O-methyl-d-glucurono)-d-xylan, with the 4-O-methyl-d-glucopyranosyluronic acid group linked to O-2 of a (1 鈫?#xA0;4)-尾-d-xylan. The repeating unit of CTPB1 is composed of one non-reducing terminal residue of 4-O-methyl-d-glucuronic acid on every four xylose residues. Immunomodulatory activity assays in vitro showed that CTPB1 may induce the proliferation of mouse splenocytes. It was suggested that CTPB1 could be a potential immunostimulant used in the food industry.