A chemical and electrochemical pre-treatment created nanopores in the screen-printed gold electrode (SPAuE) A clever electrode architecture involving a self-assembled monolayer and several multilayers Sodium dodecyl sulphonate (SDS) and nickel(II) phthalocyanine-tetrasulfonic acid were place by layer-by-layer technique An amperometric analytical response in a flow system is obtained for 2-nitro-p-phenylenediamine