Pituitary tumor transforming gene and insulin-like growth factor 1 receptor expression and immunohistochemical measurement of Ki-67 as potential prognostic markers of pituitary tumors aggressiveness

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• 作者：Laura S谩nchez-Tejada ; Ruth S谩nchez-Ortiga ; 脫scar Moreno-P茅rez ; Carmen Fajardo Monta帽ana ; Maria Niveiro ; Nicholas A. Tritos ; Antonio M. Pic贸 Alfonso
• 关键词：PA ; pituitary adenomas ; PTTG ; pituitary tumor transforming gene ; IGF1R ; insulin-like growth factor 1 receptor ; RT-PCR ; real time polymerase chain reaction ; PI3K/Akt ; phosphoinositide 3-kinase/Akt pathway ; MAPK ; mitogen-activated protein kinases ; MRI ; magnetic resonance imaging ; FFPE ; formalin-fixes and paraffin-embedded ; FPA ; functioning pituitary adenoma ; NFPA ; non-functioning pituitary adenoma ; PRL ; prolactin ; ACTH ; adrenocorticotropic hormone ; GH ; growth hormone ; TSH ; Thyroid stimulating horm
• 刊名：Endocrinolog篓陋a y Nutrici篓庐n (English Edition)
• 出版年：August-September, 2013
• 年：2013
• 卷：60
• 期：7
• 页码：358-367
• 全文大小：380 K

文摘

Introduction and objective

The ability to predict recurrence of pituitary adenoma (PA) after surgery may be helpful to determine follow-up frequency and the need for adjuvant treatment. The purpose of this study was to assess the prognostic capacity of pituitary tumor transforming gene (PTTG), insulin-like growth factor 1 receptor (IGF1R), and Ki-67.

Materials and methods

<p>In this retrospective study, the normalized copy number (NCN) of PTIG and IGF1R mRNA was measured using RT-PCR, and the Ki-67 index was measured by immunohistochemistry in 46 PA samples. Clinical data, histological subtype, and radiographic characteristics were collected to assess associations between variables and tumor behavior. Progression of tumor remnants and its association to markers was also studied in 14 patients with no adjuvant treatment after surgery followed up for 46 卤 36 months.

Results

<p>Extrasellar tumors had a lower PTTG expression as compared to sellar tumors (0.065 [1st-3rd quartile: 0.000-0.089] NCN vs. 0.135 [0.105-0.159] NCN, p = 0.04). IGF1R expression changed depending on histological subtype (p = 0.014), and was greater in tumor with remnant growth greater than 20% during follow-up (10.69 卤 3.84 NCN vs. 5.44 卤 3.55 NCN, p = 0.014).

Conclusions

<p>Our results suggest that the IGF1R is a more helpful molecular marker than PTTG in PA management. Ki-67 showed no association to tumor behavior. However, the potential of these markers should be established in future studies with standardized methods and on larger samples.