Demonstration of T cell and macrophage progenitors in carp (Cyprinus carpio) kidney hematopoietic tissues. Development of clonal assay system for carp hematopoietic cells

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• 作者：Fumihiko Katakura ; Takuya Yamaguchi ; Miyuki Yoshida ; Tadaaki Moritomo ; Teruyuki Nakanishi
• 关键词：Carp ; Cyprinus carpio ; Kidney ; Hematopoiesis ; Cell culture ; Progenitor ; Monocyte/macrophage ; T cell
• 刊名：Developmental and Comparative Immunology
• 出版年：2010
• 出版时间：June 2010
• 年：2010
• 卷：34
• 期：6
• 页码：685-689
• 全文大小：451 K

文摘

Single hematopoietic cells from carp (Cyprinus carpio) kidney were seeded to each well of 96-well plates and cultured in the presence of a supporting cell layer and conditioned media (CM). The CM were obtained from bulk-cultured carp hematopoietic cells, in which T and macrophage-lineage cells rapidly proliferated as previously reported. After 2–3 weeks, colony formation was found in 0–4 wells of each plate. Three different morphological types of colonies were observed: “type I colonies”, “type II colonies” and “mixed-type colonies”. Type I colony cells were interpreted as composed by macrophage-lineage cells, since they expressed a specific macrophage marker, M-CSFR/csf1r gene, and most of them phagocytosed latex particles. Type II colony cells were interpreted as composed by T lineage cells, since they expressed several T cell marker genes including gata3, lck and TCRβ, but did not engulf latex particles. Mixed-type colonies were interpreted as composed by both macrophages and T lineage cells. They expressed not only the M-CSFR gene but also a T cell marker gene, gata3, but not other T cell markers, such as lck and TCRβ. These results indicated that the mixed-type colonies were developed from immature common progenitors of macrophage and T cell. In contrast, type I and type II colonies were developed from more mature and mono-potent progenitors of macrophage and T cell, respectively.