Rapid quantitation of human epididymis protein 4 in human serum by amplified luminescent proximity homogeneous immunoassay (AlphaLISA)
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- 作者：Hui Zhaoa ; 1 ; Guanfeng Linb ; 1 ; Tiancai Liuc ; Junyu Liangc ; Zhiqi Renc ; Rongliang Liangc ; Baihong Chenb ; Wenhua Huanga ; huangwenhua2009@139.com" class="auth_mail" title="E-mail the corresponding author ; Yingsong Wuc ; wg@smu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：AlphaLISA ; amplified luminescent proximity homogeneous immunoassay ; HE4 ; human epididymis protein 4 ; BSA ; bovine serum albumin ; MES ; 4-morpholineethanesulfonic acid ; CMO ; carboxy-methoxy lamine ; NHS ; n-hydroxysulfosuccinimide ; SA-D beads ; streptavidincoated donor beads ; HEPES ; 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid ; EDC ; 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride ; Eu ; europium ; ECLIA ; electrochemiluminescence immunoassay ; CV ; coefficient of variation ; SD ; stand
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：437
- 期：Complete
- 页码：64-69
- 全文大小：555 K
文摘
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A homogeneous reaction measurement method bases on AlphaLISA has excellent performance.
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A new approach for the determination of HE4 was provided.
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Validation of methodology was carried out.
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20">Application was demonstrated in practical samples, with satisfactory results.