Hemozoin-free Plasmodium falciparum mitochondria for physiological and drug susceptibility studies

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• 作者：Michael W. Mather ; Joanne M. Morrisey ; Akhil B. Vaidya
• 关键词：Cell fractionation ; Mitochondria ; Malaria ; Hemozoin ; Magnetic separation ; Spectrophotometric enzyme assay
• 刊名：Molecular and Biochemical Parasitology
• 出版年：2010
• 出版时间：December 2010
• 年：2010
• 卷：174
• 期：2
• 页码：150-153
• 全文大小：372 K

文摘

Isolation of mitochondria of high purity and with intact enzymatic activities from malaria parasites has proven to be a major obstacle in characterizing the parasite mitochondrial physiology. We describe here an improved procedure for the isolation of a mitochondrially enriched preparation from the trophozoite stage of erythrocytic Plasmodium falciparum, combining disruption by N₂ cavitation and differential centrifugation with magnetic removal of hemozoin-associated material. These mitochondrial preparations may be used to assay various mitochondrial enzyme activities, such as succinate and dihydroorotate dehydrogenases, ubiquinol-cytochrome c oxidoreductase, and cytochrome c oxidase. They also exhibit a low level of ATPase activity, which is only marginally inhibited by classical inhibitors. We have used this preparation to determine the susceptibility of mitochondrial activities to drugs and drug candidate compounds in both “wild type” and transgenic parasites.