Synergistic effects of pemetrexed and amrubicin in non-small cell lung cancer cell lines: Potential for combination therapy

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• 作者：Yukihisa Hatakeyama ; Kazuyuki Kobayashi ; Tatsuya Nagano ; Daisuke Tamura ; Masatsugu Yamamoto ; Motoko Tachihara ; Yoshikazu Kotani ; Yoshihiro Nishimura
• 关键词：Non-small cell lung cancer ; Thymidylate synthase ; Pemetrexed ; Amrubicin
• 刊名：Cancer Letters
• 出版年：1 February, 2014
• 年：2014
• 卷：343
• 期：1
• 页码：74-79
• 全文大小：1685 K

文摘

The purpose is to examine the synergistic effect of pemetrexed (PEM) and amrubicin (AMR) on the proliferation of lung cancer cell lines. In vitro, dose-dependent synergistic effects of concurrent PEM and AMRol, which is an active metabolite of AMR were observed in A549 and H460 cells. In real-time RT-qPCR analysis and western blotting, expression of the target enzymes of PEM were suppressed in cells treated with amrubicinol alone. In vivo, AMR/PEM treatment also showed synergistic antitumor activity both in A549-bearing and H520-bearing mice. PEM and AMR work synergistically to inhibit the proliferation of several different lung cancer cell lines.