Compromised potassium recycling in the cochlea contributes to conservation of endocochlear potential in a mouse model of age-related hearing loss
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- 作者：Haidi Yang ; Hao Xiong ; Qiuhong Huang ; Jiaqi Pang ; Xuqing Zheng ; Lian Chen ; Rongjun Yu ; Yiqing Zheng
- 关键词：Stria vascularis ; Endocochlear potential ; Potassium transport ; C57BL/6 ; Presbycusis
- 刊名：Neuroscience Letters
- 出版年：25 October, 2013
- 年：2013
- 卷：555
- 期：Complete
- 页码：97-101
- 全文大小：1318 K
文摘
The C57BL/6 strain is considered an excellent model to study age-related hearing loss (AHL). Aging C57BL/6 mice are characterized by profound hearing loss but conservation of the endocochlear potential (EP). Here we show 12-month-old C57BL/6 mice display a notable hearing loss at 4, 8, 16 and 32 kHz while the EP is maintained at normal level. Morphological examination shows significant outer hair cells loss in the cochlear basal turn and atrophy of the stria vascularis (SV). Fluorescence immunohistochemical studies reveal that potassium channel KCNJ10 and KCNQ1 expression dramatically decreased in the SV. Concomitant with this, mRNA levels of KCNJ10 and KCNQ1 are also reduced. In addition, three other potassium transporters, including 伪1-Na,K-ATPase, 伪2-Na,K-ATPase and NKCC1, reduce their expression at mRNA levels as well. These observations suggest that conservation of the EP in aging C57BL/6 mice is attributable to the SV generating a new balance for potassium influx and efflux at a relatively lower level.